Ribo-96
Ribo96: High-Throughput Ribosome Profiling in 96-Well Format
At EIRNABio, we pride ourselves on our abilities to keep pushing back the boundaries of what is possible in the field of Translatomics. In this regard our Ribo96 service represents a quantum leap forward by enabling a high-throughput Ribosome Profiling service in a 96-well format. This significant milestone in in the field empowers researchers to gain unparalleled insights into protein synthesis faster and more affordably than ever before.
Advancing Translation Research with Ribo96
In early-stage drug discovery and preclinical development, understanding translational regulation at scale is crucial. Ribo96 revolutionizes Ribosome Profiling by enabling the simultaneous analysis of 96 samples, providing high-resolution data on active translation across multiple conditions, replicates, or treatments in a single run. This service is ideal for drug screening, cohort studies, and systems biology, democratizing access to cutting-edge translation analysis for academic and industry labs alike.
What is Ribo96?
Ribo96 maps active translation by sequencing ribosome-protected mRNA fragments at nucleotide resolution across 96 samples simultaneously. Leveraging EIRNABio’s proprietary workflows, this service slashes per-sample costs and minimizes batch effects, ensuring robust and reproducible data. By offering a scalable and affordable approach, Ribo96 bridges the gap between traditional transcriptomics and proteomics, revealing critical insights into translational efficiency and regulation.
Key Features of Ribo-96
- Scalable Analysis: Study 96 conditions, replicates, or treatments in one run, making it perfect for high-throughput drug screens or large-scale studies.
- Insightful Data: Uncover translational efficiency, ribosome stalling, upstream ORFs (uORFs), and novel ORFs to provide insights unattainable through transcriptomics or proteomics alone.
- Cost-Effective: Lower per-experiment costs compared to traditional Ribo-seq, making high-resolution translation analysis more accessible.
- Quality Assured: Rigorous quality control ensures triplet periodicity and reliable data, backed by our expertise from hundreds of Ribo-seq projects.
Challenges associated with Ribosome Profiling
Data Generation
Challenges
Technical Complexity: Regular ribosome profiling is an extremely complex and labor-intensive process. These issues are amplified when trying to run 96 samples in parallel whilst maintaining data consistency and minimizing variability
Resource Intensity: High-throughput experimentation demands specialized equipment and complex protocols, making in-house implementation costly and technically challenging for many labs, especially when scaling to large sample numbers.
High Data Volume: Handling data from 96 samples generates significant computational and analytical demands, requiring powerful computing infrastructure and specialized bioinformatics expertise to manage, process, and interpret results effectively.
Our Solutions
Expertise: Led by translatomics pioneers, EIRNA Bio has developed robust proprietary automation protocols and workflows that ensure consistent, reliable, reproducible, high-quality data in each run.
Tap into our infrastructure: We’ve invested very heavily in our people and our equipment and developed proprietary automated workflows which you can immediately leverage and remove barriers to ribosome profiling at scale to address diverse research needs.
Scalable Bioinformatics Platform: Our proprietary bioinformatics platform EIRNABio-Connect is ideally suited to comprehensively analyze data from large sample numbers and is set up to enable easy comparison between individual samples and groups of samples
Data Analysis
Challenges
Data Interpretation Complexity: Ribo96 produces rich, large-scale translational datasets that can be challenging to interpret, requiring intuitive tools to navigate complex outputs like gene expression shifts, ribosome dynamics, and codon-level behavior.
Data Overload: Extracting meaningful translational insights from 96-sample datasets demands customized bioinformatics pipelines capable of managing scale, resolving complexity, and delivering focused, actionable results for diverse experimental questions.
Solutions
EIRNA Bio-Connect Platform: Our browser-based platform streamlines Ribo96 data exploration with interactive visualizations, nucleotide-level resolution, and built-in tools for a plethora of different analyses types – supported by tutorials, help pages, and expert consultations.
Tailored Analytical Protocols: We recognize these challenges and have optimized EIRNABio-Connect to handle large sample numbers and associated data. This enables researchers to efficiently extract precise insights—comparing conditions, identifying patterns, or visualizing codon-level dynamics.
Applications of Ribo-96
- Drug Screening: Assess translational responses to 96 different compounds or conditions in a single experiment, accelerating early-stage drug discovery.
- Cohort Studies: Analyze translational profiles across large sample sets to identify biomarkers or disease mechanisms.
- Systems Biology: Gain a holistic view of translational regulation under various conditions, enhancing understanding of cellular dynamics.
- Personalized Medicine: Explore translational signatures in patient-derived samples to support tailored therapeutic strategies.
- Translational Efficiency: Quantify ribosome occupancy to understand protein synthesis rates across diverse experimental setups.
- Ribosome Pausing and uORF Regulation: Identify regulatory elements influencing translation at scale.
Ribo96 vs Traditional Ribosome Profiling
- Ribo96 offers the same nucleotide-level resolution as traditional Ribosome Profiling but scales up to 96 samples, providing unmatched throughput for large studies.
- Traditional Ribo-seq focuses on smaller sample sets, while Ribo96 is optimized for high-throughput applications like drug screens, reducing cost and time per sample.
- Both methods reveal ribosome stalling and codon-specific regulation, but Ribo96’s automation minimizes variability across large batches.
Ribo-96 Services FAQ
Ribo96 is a high-throughput Ribosome Profiling service that maps active translation across 96 samples simultaneously by sequencing ribosome-protected mRNA fragments. This provides a detailed view of protein synthesis at nucleotide resolution.
While traditional Ribosome Profiling analyzes fewer samples per run, Ribo96 scales up to 96 samples, offering a cost-effective and efficient solution for large-scale studies without compromising data quality.
Ribo96 is ideal for drug screening, cohort studies, systems biology, personalized medicine, and exploring translational efficiency and regulation on a large scale.
Ribo96 accelerates drug discovery by enabling high-throughput screening of translational responses, identifying novel targets, and optimizing protein expression for biotherapeutics.
Ribo96 can be applied to a wide range of sample types, including cell lines and tissues, with protocols tailored to specific project needs.
We offer end-to-end support, including flexible experimental design, appropriate sequencing depth, and comprehensive bioinformatics analysis via EIRNA Bio-Connect.
Contact us to schedule a meeting with our experts to discuss your project needs.